Publications

Garske AL, Oliver SS, Wagner EK, Musselman CA, LeRoy G, Garcia BA, Kutateladze TG, and Denu JM.  Combinatorial profiling of chromatin-binding modules reveals multi-site discrimination. Nature Chemical Biology 2010 Apr;6(4):283-90. (PMCID: PMC2922993, Publ.ID: NCHEMB-A090503857).

Someya S, Yu W, Hallows WC, Xu J, Vann JM, Leeuwenburgh, C, Tanokura M, *Denu JM and *Prolla TA. Mitochondrial Sirt3 Mediates Reduction of Oxidative Damage and Prevention of Age-related Hearing Loss in Response to Caloric Restriction. Cell, 143, 802-812. 2010. (*corresponding authors)

Kolonko EM, Albaugh BN, Lindner SE, Chen Y, Satyshur KA, Arnold KM, Kaufman PD, Keck JL, and Denu JM. Catalytic Activation of Histone Acetyltransferase Rtt109 by a Histone Chaperone. Proc Natl Acad Sci USA. 2010, 07(47):20275-80. PMCID: PMC2996700

Smith BC, Settles B, Hallows WC, Craven MW, Denu JM. SIRT3 Substrate Specificity Determined by Peptide Arrays and Machine Learning. ACS Chem Biol. 2011 Feb 18;6(2):146-57. PMCID: PMC3042044

Hallows, WC, Wei , Y, Smith, BC, Devries, MK, Ellinger, JJ, Someya, S, Shortreed, MR, Prolla, T, Markley, JL, Smith, LM, Zhao, S, Guan, K-L, Denu, JM. Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction. Molecular Cell, 41, 139-149, 2011. PMCID: PMC3101115

 Pan PW, Feldman JL, Devries MK, Dong A, Edwards AM, Denu JM. Structure and Biochemical Functions of SIRT6. J Biol Chem. 2011 Mar 1. PMCID: PMC3077655

Chen D, Vollmar M, Rossi MN, Phillips C, Kraehenbuehl R, Slade D, Mehrotra PV, von Delft F, Crosthwaite SK, Gileadi O, *Denu JM, *Ahel I.  Identification of macro domain proteins as novel O-acetyl-ADP-ribose deacetylases. J Biol Chem. 2011 Jan 21. (*corresponding authors). PMCID: PMC3075673

Smith BC, Hallows WC, Denu JM. A continuous microplate assay for sirtuins and nicotinamide-producing enzymes. Anal Biochem. 2009 Nov 1;394(1):101-9. PMCID: PMC2752052.

Tong, L, Lee, S, and Denu, JM. Hydrolase regulates NAD metabolites and modulates cellular REDOX. J Biol Chem. 2009, Apr 24;284(17):11256-66. PMCID: PMC2670130.

Smith, BC, Hallows, WC, Denu, JM. Mechanisms and Molecular Probes of Sirtuins. Chemistry and Biology, 2008 Oct 20;15(10):1002-13. PMCID: PMC2626554.

Lee, S. Tong, L. and Denu, JM. Quantification of endogenous sirtuin metabolite O-acetyl-ADP-ribose. Anal Biochem. 2008 Dec 15;383(2):174-9. PMCID: PMC2586145.

Berndsen, CE, Tsubota, T, Lindner, SE, Lee, S, Holton, JM, Kaufman, PD, Keck, JL, and Denu, JM. Molecular functions of the histone acetyltransferase•chaperone complex Rtt109•Vps75. Nature Structural and Molecular Biology, 2008 Sep;15(9):948-56. PMCID: PMC2678805.

Kowieski TM, Lee S, Denu JM. Acetylation-dependent ADP-ribosylation by trypanosoma brucei sir2. J Biol Chem. Feb 2008 283(9):5317-26.

Smith, BC and Denu, JM. Mechanism-Based Inhibition of Sir2 Deacetylases by Thioacetyl-Lysine Peptide. Biochemistry. 2007, 46(50):14478-86.

Smith, BC and Denu, JM. Acetyl-lysine analog peptides as mechanistic probes of protein deacetylases. J Biol Chem. 2007, 282(51):37256-65.

Comstock, LR and Denu JM. Synthesis and biochemical evaluation of O-acetyl-ADP-ribose and N-acetyl analogs, Org. Biomol. Chem., 2007, 5(19):3087-91.

Smith, BC and Denu, JM. Sir2 Deacetylases Exhibit Nucleophilic Participation of Acetyl-lysine in NAD+ Cleavage. J Am Chem Soc. 2007 May 9;129(18):5802-3.

Denu, JM. Vitamins and Aging: Pathways to NAD+ Synthesis. Cell. 2007 May 4;129:453-454.

Grubisha, O, Rafty, LA, Takanishi, CL, Xu, X, Tong, L, Perraud, AL, Scharenberg, AM, Denu, JM.  Metabolite of SIR2 reaction modulates TRPM2 ion channel. J Biol Chem. 281(20):14057-15065 (2006).

Smith, BC and Denu, JM. Sir2 Protein Deacetylases: Evidence for Chemical Intermediates and Functions of a Conserved Histidine. Biochemistry, 45(1):272-82 (2006).

Hallows, WC, Lee, S, Denu, JM. Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.  Proc Natl Acad Sci USA. 103(27):10230-5 (2006).

Hallows WC*, Yu W*, Smith BC, Devries MK, Ellinger JJ, Someya S, Shortreed MR, Prolla T, Markley JL, Smith LM, Zhao S, Guan KL, Denu JM. (2011). Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction. Molecular Cell. Jan 21;41(2):139-49. (* equally contribution)

Someya S*, Yu W, Hallows WC, Xu J, Vann JM, Leeuwenburgh C, Tanokura M, Denu JM, Prolla TA. (2010). Sirt3 mediates reduction of oxidative damage and prevention of age-related hearing loss under caloric restriction. Cell. Nov 24;143(5):802-12. (* equally contribution)

Guan KL, Yu W, Lin Y, Xiong Y, Zhao S. Generation of acetyllysine antibodies and affinity enrichment of acetylated peptides. (2010). Nature Protocols. Sep:5(9):1583-95.

Zhao S., Xu W., Jiang W.,Yu W., Lin Y, Zhang T., Yao J., Yang P., Li H., Li Y, Shi J., Qin l, Lei Q., Xiong Y., Guan KL. (2009). Protein acetylation plays an extensive role in regulating cellular metabolism.Science. 327.5968,1000-1004.

Yu W.,Lin Y., Yao J., Huang W., Lei Q., Xiong Y., Zhao S., Guan KL. (2009). Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals.J. Bio. Chem. 284(20): 13669-13675.